Molecular characterization, tissue distribution and differential nutritional regulation of putative Elovl5 elongase in silver barb ( Puntius gonionotus )
作者: Madhusmita NayakAvinash PradhanShiba Shankar GiriMrinal SamantaV. Badireenath KonkimallaAshis Saha
作者单位: 1Division of Fish Nutrition and Physiology, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India
2Division of Fish Health Management, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India
3School of Biological Sciences, National Institute of Science Education & Research Bhubaneswar, Jatni, Khurda, Odisha, India
刊名: Comparative Biochemistry and Physiology, Part B, 2018, Vol.217 , pp.27-39
来源数据库: Elsevier Journal
DOI: 10.1016/j.cbpb.2017.12.004
关键词: Silver barbElovl 5LC-PUFA biosynthesis3D structureNutritional regulation
英文摘要: Abstract(#br)Fatty acid desaturase (Fads) and elongase (Elovl) are two important enzymes involved in the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFAs) in fishes. Silver barb ( Puntius gonionotus ) is considered a promising medium carp species for freshwater aquaculture in Asia. Earlier, Δ6 fads cDNA was characterized to understand the molecular mechanism of LC-PUFA biosynthesis in this species. In the present study elovl5 -like elongase full-length cDNA was cloned and characterized by RACE (rapid amplification of cDNA ends), and analyzed its basal expression in various organs/tissues by quantitative real time PCR (qRT-PCR) assay. Differential expression of elovl5 cDNA in various tissues following replacement of fish oil (FO) with linseed oil (LO) in the diet also...
全文获取路径: Elsevier  (合作)
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关键词翻译
关键词翻译
  • regulation 
  • differential 差动的
  • tissue 组织
  • biosynthesis 生物合成
  • Silver 
  • silver 
  • putative 假定的
  • distribution 分布
  • structure 构造
  • nutritional 营养的