Abstract(#br)Prior studies show that Borago officinalis L. (BO) can suppress lipid accumulation in 3 T3-L1 adipocytes. Similarly, we recently revealed that Erigeron annuus L. Pers (EA) can significantly diminish both lipid accumulation and adipocyte differentiation in 3 T3-L1 cells through an AMPK (AMP-activated protein kinase)-dependent mechanism. Accordingly, the objective of this present study was to evaluate the anti-obesity activity of EA and/or BO using an animal model of obesity. Obesity was induced in C57BL/6 J mice by feeding a high-fat diet (HFD; 60 kcal% fat) for 3 weeks, followed by administration of EA and/or BO (100-200 mg/kg body weight) or positive control Garcinia Cambogia (GC) (100 mg/kg body weight) for an additional 8 weeks. The anti-obesity effect of EA and/or BO was... assessed by measuring body weight, adipocyte size, lipid accumulation, and expression level of genes associated with adipogenesis. We found the administration of EA and/or BO significantly attenuated increases in body weight gain, adipocyte size, and lipid accumulation in obese mice induced by HFD. In addition, western blot analysis revealed that HFD-mediated increases in expressions levels of adipogenic genes such as PPARγ, C/EBPα, and SREBP-1c were diminished by EA and/or BO. Moreover, EA and/or BO significantly stimulated the production of adiponectin, a unique adipokine known to stimulate the breakdown of fat/lipids, whereas adiponectin levels were reduced in mice fed a HFD. Notably, a combination of EA and BO was more effective at modulating such parameters than EA or BO alone. Taken together, these results demonstrate that an anti-obesity effect of EA and/or BO can reduce adipocyte hypertrophy and modulate the expression of adipogenesis-associated genes.