Directed evolution of carbon–hydrogen bond activating enzymes
作者: Raphael FreyTakahiro HayashiRebecca M Buller
作者单位: 1Institute of Chemistry and Biotechnology, Department of Life Sciences and Facility Management, Zurich University of Applied Sciences, Einsiedlerstrasse 31, 8820 Waedenswil, Switzerland
刊名: Current Opinion in Biotechnology, 2019, Vol.60 , pp.29-38
来源数据库: Elsevier Journal
DOI: 10.1016/j.copbio.2018.12.004
原始语种摘要: As industrial biocatalysis is maturing, access to enzymatic activities beyond chiral resolutions, asymmetric ketone reductions and reductive aminations is gradually becoming reality. Especially the utilization of carbon–hydrogen bond (C–H) activating enzymes is very attractive as they catalyze a variety of chemically extremely challenging transformations. Because of their intrinsic complexity, the use of these enzymes in manufacturing has been limited. However, recent advances in enzyme engineering and bioinformatics have led to activity improvements for native and non-native substrates, the introduction of new-to-nature chemistries and the identification of promising novel enzyme families. Looking forward, the use of automation and advanced computer algorithms will help to streamline the...
全文获取路径: Elsevier  (合作)
影响因子:7.86 (2012)

  • evolution 进化
  • enzymes 酶类
  • activating 激活
  • bioinformatics 生物信息学
  • carbon 
  • biocatalysis 生物催化
  • recent 近来的
  • native 国产的
  • manufacturing 制造
  • catalyze 催化