Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates
作者: Jontana AllkjaThomas BjarnsholtTom CoenyePaul CosAdyary FallareroJoe J. HarrisonSusana P. LopesAntonio OliverMaria Olivia PereiraGordon RamageMark E. ShirtliffPaul StoodleyJeremy S. WebbSebastian A.J. ZaatDarla M. GoeresNuno Filipe Azevedo
作者单位: 1LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal
2Montana State University, Center for Biofilm Engineering, 366 Barnard Hall, Bozeman, MT, 59717, USA
3Department of Clinical Microbiology, Rigshospitalet, 2100, Copenhagen, Denmark
4Department of Immunology and Microbiology, Costerton Biofilm Center, Faculty of Health Sciences University of Copenhagen, 2200, Copenhagen, Denmark
5ESCMID Study Group for Biofilms, Basel, Switzerland
6Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium
7Laboratory for Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium
8Pharmaceutical Design and Discovery (PharmDD), Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5E, P.O. Box 56, FI-00014, Helsinki, Finland
9Department of Biological Scie
刊名: Biofilm, 2020, Vol.2
来源数据库: Elsevier Journal
DOI: 10.1016/j.bioflm.2019.100010
关键词: BiofilmReproducibilityGuidelinesMicroplateSpectrophotometryFluorometry
英文摘要: Abstract(#br)The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of...
全文获取路径: Elsevier  (合作)

  • fluorometric 荧光的
  • biofilm 生物薄膜
  • guideline 驾束
  • information 报告
  • assess 查定