Administration of Gemcitabine After Pancreatic Tumor Resection in Mice Induces an Antitumor Immune Response Mediated by Natural Killer Cells
作者: Engin GürlevikBettina Fleischmann-MundtJennifer BrooksIhsan Ekin DemirKatja SteigerSilvia RibbackTetyana YevsaNorman WollerArnold KloosDmitrij OstroumovNina ArmbrechtMichael P. MannsFrank DombrowskiMichael SaborowskiMoritz KleineThomas C. WirthHelmut OettleGüralp O. CeyhanIrene EspositoDiego F. CalvisiStefan KubickaFlorian Kühnel
作者单位: 1Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Hannover, Germany
2Department of Surgery, Hannover Medical School, Hannover, Germany
3Department of Surgery, Klinikum Rechts der Isar, Technische Universität München, Munich, Germany
4Institute of Pathology, Klinikum Rechts der Isar, Technische Universität München, Munich, Germany
5Institute of Pathology, University Medicine of Greifswald, Greifswald, Germany
6Charité University Medicine Berlin, Berlin, Germany
7Institute of Pathology, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany
8Cancer Center Reutlingen, District Hospital, Reutlingen, Germany
刊名: Gastroenterology, 2016, Vol.151 (2), pp.338-350.e7
来源数据库: Elsevier Journal
DOI: 10.1053/j.gastro.2016.05.004
关键词: Pancreatic CancerSurgeryAdjuvant TherapyResectable Transgenic Mice
英文摘要: Background & Aims(#br)Even after potentially curative R0 resection, patients with pancreatic ductal adenocarcinoma (PDAC) have a poor prognosis owing to high rates of local recurrence and metastasis to distant organs. However, we have no suitable transgenic animal models for surgical interventions.(#br)Methods(#br)To induce formation of pancreatic tumor foci, we electroporated oncogenic plasmids into pancreata of LSL-KrasG12D × p53fl/fl mice; mutant Kras was expressed in p53fl/fl mice using a sleeping beauty transposon. We co-delivered a transposon encoding a constitutively active form of Akt2 (myrAkt2). Carcinogenesis and histopathologic features of tumors were examined. Metastasis was monitored by bioluminescence imaging. Tumors were resected and mice were given gemcitabine, and tumor...
全文获取路径: Elsevier  (合作)
影响因子:12.821 (2012)