Naked pDNA/hyaluronic acid powder shows excellent long-term storage stability and gene expression in murine lungs
作者: Takaaki ItoMaino FukuharaTomoyuki OkudaHirokazu Okamoto
作者单位: 1Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya 468-8503, Japan
刊名: International Journal of Pharmaceutics, 2020, Vol.574
来源数据库: Elsevier Journal
DOI: 10.1016/j.ijpharm.2019.118880
关键词: Plasmid DNAInhalationSpray freeze-dryingPulmonary drug deliveryLung drug deliveryGene deliveryGene therapy
英文摘要: Abstract(#br)We evaluated the storage stability of powder containing naked plasmid DNA (pDNA) and hyaluronic acid (HA) or mannitol (Man) prepared by the spray-freeze-drying technique, through which we have reported high gene expression without any gene vectors. The powders composed of 5–10-µm porous particles and showing excellent dispersion were stored for 12 months under three storage conditions: 5 °C/Dry, 25 °C/Dry, and 25 °C/75% relative humidity. The humidified powders lost their porous shape within 1 week and were not suitable for inhalation characterization. On the other hand, the powders under dry conditions maintained high inhalation characteristics and pDNA integrity for 12 months. We administered the powders to the lungs of mice. The naked pDNA in HA powder showed significantly...
全文获取路径: Elsevier  (合作)
影响因子:3.458 (2012)

  • expression 表示
  • murine 鼠灰色
  • delivery 移交
  • lungs 
  • drying 干燥
  • excellent 优秀的
  • freeze 
  • DNA Deoxyribonucleic Acid
  • powder 粉末
  • therapy 治疗