Identification of proteomic markers for ram spermatozoa motility using a tandem mass tag (TMT) approach
作者: Wen ZhuYan ZhangChun-huan RenXiao ChengJia-hong ChenZe-yong GeZhi-peng SunXian ZhuoFeng-fei SunYa-le ChenXiao-jiao JiaZijun Zhang
作者单位: 1College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiangxilu Road, Hefei 230036, PR China
2New Rural Develop Research Institute, Anhui Agricultural University, 130 Changjiangxilu Road, Hefei 230036, PR China
刊名: Journal of Proteomics, 2020, Vol.210
来源数据库: Elsevier Journal
DOI: 10.1016/j.jprot.2019.103438
关键词: Sperm motilitySpermatozoaTandem mass tag (TMT)ProteomicsRam
原始语种摘要: Abstract(#br)Sperm motility is crucial for ram fertility; however, differences in the proteome of sperm with high- and low- motility in rams ( Ovis aries ) has yet to be achieved. Thus, the aim of this study was to identify and characterize ram spermatozoa proteins with different abundances between high- and low- motility, and identify of proteomic markers for ram spermatozoa motility using tandem mass tag (TMT) protein labeling and LC-MS/MS. In this study, the abundance of 150 proteins in high-motility (HM) ram sperm was significantly different compared with low-motility (LM) sperm. Proteins involved in sperm motility, mitochondrial activity, and spermatogenesis showed higher abundance in HM ram spermatozoa; proteins involved in protein processing and spliceosome were more abundant in LM...
全文获取路径: Elsevier  (合作)
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来源刊物:
影响因子:4.088 (2012)

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关键词翻译
关键词翻译
  • spermatozoa 精子
  • tandem 串联机
  • motility 游动性
  • TMT Transonic Model Tunnel
  • approach 
  • markers 时标