Characterization of functional VIP/PACAP receptors in the human erythroleukemic HEL cell line
作者: Roger Lema-KisokaNathalie HayezIngrid LangerPatrick RobberechtEric SaribanChristine Delporte
作者单位: 1Department of Biochemistry and Nutrition, Faculty of Medicine, Université Libre de Bruxelles, Brussels, Belgium
2Laboratory of Pediatric-Oncology, Hôpital Universitaire des Enfants, Brussels, Belgium
刊名: Peptides, 2001, Vol.22 (12), pp.2155-2162
来源数据库: Elsevier Journal
DOI: 10.1016/S0196-9781(01)00567-8
关键词: VIPPACAP receptorsErythroleukemiaDesensitizationCyclic AMP
原始语种摘要: Abstract(#br)R. LEMA-KISOKA, N. HAYEZ, I. LANGER, P. ROBBERECHT, E. SARIBAN AND C. DELPORTE. Characterization of functional VIP/PACAP receptors in the human erythroleukemic HEL cell line. PEPTIDES. The presence of VIP/PACAP receptors was investigated on the human erythroleukemic cell line HEL. Specific binding of [ 125 I]-PACAP or [ 125 I]-VIP on HEL cells or membranes was very low and did not allow to perform competition curves. At 37°C PACAP transiently increased cAMP levels in the presence of the non-specific phosphodiesterase inhibitor IBMX, suggesting rapid desensitization. Kinetic studies revealed that optimal conditions to measure the EC 50 of PACAP(1–27) were 10 min at 20°C. Under those conditions, PACAP-related peptides increased cAMP levels with EC 50 in agreement with the...
全文获取路径: Elsevier  (合作)
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影响因子:2.522 (2012)

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关键词翻译
关键词翻译
  • 已确认的 增加
  • functional 功能的
  • VIP Variable Information Processing
  • homologous 同结构型的
  • heterologous 异种的
  • inactive 不活泼的
  • increased 增加
  • confirmed 增加
  • expression 表示
  • cAMP 环腺苷酸
  • related 有关的