Transformation of Primary Chick Embryo Fibroblasts by Marek's Disease Virus
作者: Chantanee BuranathaiJose RodriguezCharles Grose
作者单位: 1Department of Microbiology, University of Iowa College of Medicine, Iowa City, Iowa, 52242
刊名: Virology, 1997, Vol.239 (1), pp.20-35
来源数据库: Elsevier Journal
DOI: 10.1006/viro.1997.8854
原始语种摘要: Abstract(#br)Marek's disease virus (MDV) is an alphaherpesvirus, which can mediate the malignant transformation of lymphocytes to form lymphomas in chickens. In this study, we demonstrate that MDV can transform primary chick embryo fibroblasts (CEF). The cell line derived from primary CEF infected with the GA strain of MDV was called CEM (MDV) . The fibroblast nature of CEM (MDV) was verified by absence of cytokeratin type II. The CEM (MDV) phenotype differed from either primary CEF or MDV-infected CEF. CEM (MDV) were extensively vacuolated, with unusual multilamellar structures in the cytoplasm. The nuclei were considerably larger than those in primary CEF and were uniformly positive for proliferating cell nuclear antigen. The cell line was subcultured for more than 10 generations;...
全文获取路径: Elsevier  (合作)
影响因子:3.367 (2012)

  • demonstrated 探明的储量
  • transformation 变换
  • CEF Common Equipment Frame
  • infectivity 传染性
  • fully 充分
  • glycoprotein 糖蛋白
  • detected 找到
  • infected 被感染
  • addition 添加
  • either 任何