Crosslinked, cryostructured Lactobacillus reuteri monoliths for production of 3-hydroxypropionaldehyde, 3-hydroxypropionic acid and 1,3-propanediol from glycerol
作者: Oksana ZaushitsynaTarek DishishaRajni Hatti-KaulBo Mattiasson
作者单位: 1Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden
2Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, 62511 Beni-Suef, Egypt
刊名: Journal of Biotechnology, 2017, Vol.241 , pp.22-32
来源数据库: Elsevier Journal
DOI: 10.1016/j.jbiotec.2016.11.005
关键词: Cryogel monolithLactobacillus reuteriGlycerolFlux analysisCell immobilizationCrosslinking
原始语种摘要: Abstract(#br)Crosslinked, cryostructured monoliths prepared from Lactobacillus reuteri cells were evaluated as potential immobilized whole-cell biocatalyst for conversion of glycerol, to potentially important chemicals for the biobased industry, i.e. 3-hydroxypropionaldehyde (3HPA), 3-hydroxypropionic acid (3HP) and 1,3-propanediol (1,3PDO). Glutaraldehyde, oxidized dextran and activated polyethyleneimine/modified polyvinyl alcohol (PEI/PVA) were evaluated as crosslinkers; the latter gave highly stable preparations with maintained viability and biocatalytic activity. Scanning electron microscopy of the PEI/PVA monoliths showed high density of crosslinked cells with wide channels allowing liquid flow through. Flux analysis of the propanediol-utilization pathway, incorporating glycerol/diol...
全文获取路径: Elsevier  (合作)
影响因子:3.183 (2012)

  • glycerol 甘油
  • propanediol 丙二醇
  • reactor 反应器
  • monolith 单成岩
  • production 生产
  • continuous 连续的
  • operation 运算
  • immobilization 固定
  • operated 被操作的
  • conditions 条件式