Flow cytometry and FACS applied to filamentous fungi
作者: Robert-Jan BleichrodtNick D. Read
作者单位: 1Manchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, M13 9NT, Manchester, United Kingdom
刊名: Fungal Biology Reviews, 2018
来源数据库: Elsevier Journal
DOI: 10.1016/j.fbr.2018.06.001
关键词: FACSFilamentous fungiFlow cytometryFluorescenceMicrocolonySingle cell
英文摘要: Abstract(#br)Flow cytometry is an automated, laser- or impedance-based, high throughput method that allows very rapid analysis of multiple chemical and physical characteristics of single cells within a cell population. It is an extremely powerful technology that has been used for over four decades with filamentous fungi. Although single cells within a cell population are normally analysed rapidly on a cell-by-cell basis using the technique, flow cytometry can also be used to analyse cell ( e.g. spore) aggregates or entire microcolonies. Living or fixed cells can be stained with a wide range of fluorescent reporters to label different cell components or measure different physiological processes. Flow cytometry is also suited for measurements of cell size, interaction, aggregation or shape...
全文获取路径: Elsevier  (合作)
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关键词翻译
关键词翻译
  • filamentous 丝状的
  • FACS Floating decimal Abstract Coding System
  • fungi 真菌
  • applied 应用的
  • cytometry 血细胞计数