Comparative assessment between objective and subjective methods in slides stained by immunohistochemistry
作者: Fernando de Andrade Quintanilha RibeiroCelina Siqueira Barbosa PereiraRicardo Junchen ChiPatrícia Lumi YokomizoJosé Humberto Tavares Guerreiro FregnaniRafael Malagoli Rocha
作者单位: 1PhD; Professor, Department of Otorhinolaryngology, School of Medical Sciences of Santa Casa de São Paulo
2PhD; Adjunct Professor, Department of Morphology, School of Medical Sciences of Santa Casa de São Paulo
36 th -year Medical Student, School of Medical of Sciences of Santa Casa de São Paulo; Resident Physician
43 rd -year Medical Student, School of Medical Sciences of Santa Casa de São Paulo
5PhD, Researcher at the Researcher Support Core of the Institute of Education and Research of the Barretos Cancer Hospital
6PhD, Research Scientist, Department of Pathology, AC Camargo Hospital - São Paulo. School of Medical Sciences of the Santa Casa de São Paulo
刊名: Brazilian Journal of Otorhinolaryngology, 2013, Vol.79 (6), pp.704-708
来源数据库: Elsevier Journal
DOI: 10.5935/1808-8694.20130129
关键词: biomedical technology evaluationcholesteatomacompared histologyimmunohistochemistry
原始语种摘要: Abstract(#br)Objective methods of assessment are often required in scientific studies. Histological tests with immunohistochemical staining can be assessed by photometry.(#br)Objective(#br)To compare this objective method with the subjective evaluation performed by three independent examiners, using slides of acquired middle ear cholesteatomas.(#br)Method(#br)We selected a total of 54 cholesteatoma images, immunohistochemically stained by anti-TNF-R2 (32 slides) and anti-TGF-α, (22 slides). The secondary antibody used in the two groups was the Max Polymer Detection System (Novo Link Kit, Novocastra®, UK). The samples were processed by a digital slide scanner (ScanScope - Aperio). The selected sites were analyzed by photometry.(#br)Results(#br)The objective assessment by photometry was...
全文获取路径: Elsevier  (合作)
影响因子:0.545 (2012)

  • cholesteatoma 胆脂瘤
  • assessment 评价
  • slides 幻灯片
  • subjective 舟的
  • objective 接物镜
  • stained 污损
  • photometry 光度测定法
  • compared 肿瘤和正常组织细胞周期的比较
  • moderate 适当的
  • between 在中间