Improving efficiency of viability-PCR for selective detection of live cells
作者: Esther Nkuipou-KenfackHolger EngelSarah FakihAndreas Nocker
作者单位: 1Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK
2QIAGEN GmbH, Hilden, QIAGEN Strasse 1, 40724 Hilden, Germany
3Cranfield Water Science Institute, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK
刊名: Journal of Microbiological Methods, 2013, Vol.93 (1), pp.20-24
来源数据库: Elsevier Journal
DOI: 10.1016/j.mimet.2013.01.018
关键词: Propidium monoazideViability PCRLive-dead distinctionMembrane integritySodium deoxycholate
原始语种摘要: Abstract(#br)Viability PCR (v-PCR) as a method to selectively detect intact live cells has gained considerable interest over the last years with an increasing number of applications. The principle is based on treatment of microbiological samples with a viability dye prior to extraction of genomic DNA and its amplification. The dye is selectively taken up by membrane-compromised dead cells resulting in the degradation of their DNA upon light exposure and therefore inhibition of amplification. Although the treatment greatly helps to generate more meaningful data, one of the main drawbacks of the technique is currently that the exclusion of dead cell signals can be incomplete leading to false-positive signals. The resulting overestimation of live cell population is especially problematic for...
全文获取路径: Elsevier  (合作)
影响因子:2.161 (2012)

  • efficiency 效率
  • cells 麻风细胞
  • treatment 处理
  • undesired 不希望有的
  • degradation 减嚣夷酌
  • PCR Page Control Register
  • relationship 关系
  • overestimation 估计过高
  • PMA Physical Memory Address
  • considerable 相当