Assessment of five soil DNA extraction methods and a rapid laboratory-developed method for quality soil DNA extraction for 16S rDNA-based amplification and library construction
作者: Kalpana SagarSalam Pradeep SinghKapil Kumar GoutamBolin Kumar Konwar
作者单位: 1Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, 784028, Assam, India
刊名: Journal of Microbiological Methods, 2014, Vol.97 , pp.68-73
来源数据库: Elsevier Journal
DOI: 10.1016/j.mimet.2013.11.008
关键词: Humic substancesChimericMetagenomic DNA
原始语种摘要: Abstract(#br)Extraction of DNA from soil samples using standard methods often results in low yield and poor quality making them unsuitable for community analysis through polymerase chain reaction (PCR) due to the formation of chimeric products with smaller template DNAs and the presence of humic substances. The present study focused on the assessment of five different methods for metagenomic DNA isolation from soil samples on the basis of processing time, purity, DNA yield, suitability for PCR, restriction digestion and mDNA library construction. A simple and rapid alkali lysis based on indirect DNA extraction from soil was developed which could remove 90% of humic substances without shearing the DNA and permits the rapid and efficient isolation of high quality DNA without the requirement...
全文获取路径: Elsevier  (合作)
影响因子:2.161 (2012)

  • quality 品质
  • construction 构造
  • DNA Deoxyribonucleic Acid
  • library 图书馆
  • humic 腐殖的
  • amplification 放大
  • extraction 提取
  • digestion 消化
  • lysis 分解
  • based 基于