Optimization of energy transfer between quantum dots and gold nanoparticles in head-to-head configuration for detection of fusion gene
作者: Taegyeong KangHyun Chang KimSang-Woo JooSo Yeong LeeIk-Sung AhnKyong-Ah YoonKangtaek Lee
作者单位: 1Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Republic of Korea
2Department of Chemistry, Soongsil University, Seoul, Republic of Korea
3Laboratory of Veterinary Pharmacology, Seoul National University, Seoul, Republic of Korea
4National Cancer Center, Goyang, Gyeonggi, Republic of Korea
刊名: Sensors & Actuators: B. Chemical, 2013, Vol.188 , pp.729-734
来源数据库: Elsevier Journal
DOI: 10.1016/j.snb.2013.07.081
关键词: EML4–ALKFusion geneSimultaneous detectionQuantum dotsGold nanoparticlesEnergy transfer
原始语种摘要: Abstract(#br)We report simultaneous detection of the variants 3a and 3b in the oncogenic EML4–ALK fusion gene using cDNA from lung cancer patients based on energy transfer between CdSe/ZnS quantum dots (QDs) and gold nanoparticles (AuNPs). To overcome the limitations of the conventional detection methods based on energy transfer, we have applied a head-to-head configuration of QDs and AuNPs. This allowed us not only to use longer probe oligonucleotides than the conventional methods, but also to optimize interparticle distance between QDs and AuNPs for maximum sensitivity and selectivity. By functionalizing two types of QDs (i.e., green- and red-emitting QDs) and 5nm AuNPs with probe oligonucleotides, we demonstrated that energy was selectively transferred from...
全文获取路径: Elsevier  (合作)
影响因子:3.535 (2012)

  • detection 探测
  • configuration 配置
  • energy 能量
  • transfer 转换
  • fusion 融解
  • selectivity 选择性
  • fluorescence 荧光
  • between 在中间
  • EML Emulator Machine Language
  • emitting 发出