Bisulfite as scavenger for enhanced biotechnological production of 3-hydroxypropionaldehyde by Lactobacillus reuteri
作者: Marc J.A. StevensSabine VollenweiderPeter MertesChristophe Lacroix
作者单位: 1Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zürich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland
刊名: Biochemical Engineering Journal, 2013, Vol.79 , pp.239-245
来源数据库: Elsevier Journal
DOI: 10.1016/j.bej.2013.08.002
关键词: In situ product removalReuterinGlycerol
原始语种摘要: Abstract(#br)Lactobacillus reuteri converts glycerol into 3-hydroxypropionaldehyde (3-HPA), a promising preservative and chemical precursor. Production of 3-HPA by L. reuteri is limited due to the toxicity of 3-HPA to the producing cells. To circumvent this toxicity, the suitability of bisulfite as 3-HPA scavenger during production was assessed. Concentrations of 400mM 3-HPA or bisulfite caused a viable cell number decrease from 10 10 to 10 7 and 10 8 cells/ml, respectively, in 1h. In contrast, the 3-HPA-bisulfite adduct was not toxic for the cells.(#br)Cells exhibiting high specific 3-HPA yield in a standardized glycerol conversion test were used to test bisulfite as scavenger during 3-HPA production. Addition of bisulfite (pH 5.8)...
全文获取路径: Elsevier  (合作)
影响因子:2.579 (2012)

  • bisulfite 亚硫酸氢盐
  • scavenger 食腐动物
  • production 生产
  • glycerol 甘油
  • suitability 适应
  • cells 麻风细胞
  • virtually 实际上
  • enhanced 增强
  • circumvent 巧妙躲避
  • preservative 防腐剂