Improvement of NADPH-dependent P450-mediated biotransformation of 7α,15α-diOH-DHEA from DHEA by a dual cosubstrate-coupled system
作者: Yan WuHui LiXiao-Mei ZhangJin-Song GongHeng LiZhi-Ming RaoJin-Song ShiZheng-Hong Xu
作者单位: 1School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, People’s Republic of China
2The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, People’s Republic of China
刊名: Steroids, 2015, Vol.101 , pp.15-20
来源数据库: Elsevier Journal
DOI: 10.1016/j.steroids.2015.05.005
关键词: DHEABiotransformationNADPHColletotrichum lini
原始语种摘要: Abstract(#br)Hydroxylation of DHEA to 7α,15α-diOH-DHEA was catalyzed by NADPH-dependent cytochrome P450 monooxygenase from Colletotrichum lini . By adding coenzyme precursor nicotinic acid, the NADPH/NADP ratio was significantly increased, and the 7α,15α-diOH-DHEA molar conversion was enhanced from 37.37% to 50.85%. To improve the availability of intracellular NADPH, a dual cosubstrate-coupled system consisting of nicotinic acid and glucose was investigated in C. lini . Using 20mM nicotinic acid and 15g/L glucose as cosubstrate for NADPH regeneration, the 7α,15α-diOH-DHEA molar conversion was dramatically increased by 74.58%. The conversion course was simultaneously shortened by 30h. Moreover, a fed-batch transformation model was...
全文获取路径: Elsevier  (合作)
影响因子:2.803 (2012)

  • cosubstrate 共底物
  • coupled 耦合
  • NADPH 还原型烟酰胺腺嘌呤二核苷酸磷酸
  • dependent 从属的
  • system