Biotransformation of glycerol to 3-hydroxypropionaldehyde: Improved production by in situ complexation with bisulfite in a fed-batch mode and separation on anion exchanger
作者: Roya R.R. SardariTarek DishishaSang-Hyun PyoRajni Hatti-Kaul
作者单位: 1Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden
刊名: Journal of Biotechnology, 2013, Vol.168 (4), pp.534-542
来源数据库: Elsevier Journal
DOI: 10.1016/j.jbiotec.2013.09.009
关键词: 3HPA-bisulfite complexIn situ recoveryAnion exchange resinAdsorption isothermColumn separation
原始语种摘要: Abstract(#br)3-Hydroxypropionaldehyde (3HPA) is an important C3 chemical that can be produced from renewable glycerol by resting whole cells of Lactobacillus reuteri . However the process efficiency is limited due to substrate inhibition, product-mediated loss of enzyme activity and cell viability, and also formation of by-products. Complex formation of 3HPA with sodium bisulfite and subsequent binding to Amberlite IRA-400 was investigated as a means of in situ product recovery and for overcoming inhibition. The adsorption capacity and -isotherm of the resin were evaluated using the Langmuir model. The resin exhibited maximum capacity of 2.92mmol complex/g when equilibrated with 45mL solution containing an equilibrium mixture of 2.74...
全文获取路径: Elsevier  (合作)
影响因子:3.183 (2012)

  • bisulfite 亚硫酸氢盐
  • glycerol 甘油
  • complexation 络合作用
  • separation 分选
  • resin 
  • batch 一批
  • isotherm 等温线
  • capacity 电容量
  • Adsorption 吸附
  • binding 装订