Eriochrome Black T inhibits endothelial cell growth through S-phase blockade
作者: Ingrid LangerGhanem AtassiPatrick RobberechtAnne Résibois
作者单位: 1Department of Biochemistry and Nutrition, Faculty of Medicine, Université Libre de Bruxelles, Brussels, Belgium
2Department of Fundamentals Physiology and Pharmacology, Institute of Pharmacy, Université Libre de Bruxelles, Brussels, Belgium
3Institut de Recherches Internationales Servier, Service cancérologie, Suresnes, France
刊名: European Journal of Pharmacology, 2000, Vol.399 (2), pp.85-90
来源数据库: Elsevier Journal
DOI: 10.1016/S0014-2999(00)00370-8
关键词: SuraminEriochrome Black TEndothelial cellCyclinCell cycle
原始语种摘要: Abstract(#br)We used human umbilical vein endothelial cells (HUVEC) cultures to investigate in vitro the antiproliferative effects of suramin and of its analogue, Eriochrome Black T. The cell cycle phases of interest were characterised with specific immune sera raised against cyclin D 1 , cyclin E and proliferating nuclear cell antigen (PCNA). Simultaneous detection of two cell cycle markers was ensured by double colour immunofluorescence. Both compounds inhibited the endothelial cell growth while Eriochrome Black T was more potent than suramin. Suramin induced HUVEC to accumulate in G1-phase as an increase of the number of cells expressing both cyclin D 1 and PCNA was observed. Eriochrome Black T preferentially blocked them in the early S-phase, as it increased the proportion of cyclin E...
全文获取路径: Elsevier  (合作)
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影响因子:2.592 (2012)

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关键词翻译
关键词翻译
  • suramin 苏拉明
  • Black 布莱克
  • endothelial 内皮的
  • blockade 封闭
  • growth 生长
  • cyclin 细胞周期蛋白
  • umbilical 供应脐带
  • accumulate 堆积
  • phase 相位
  • potent 强大的