CXCR4 and CXCR7 play distinct roles in cardiac lineage specification and pharmacologic β-adrenergic response
作者: Delaine K. CeholskiIrene C. TurnbullVenu PothulaLaura LecceAndrew A. JarrahChangwon KhoAhyoung LeeLahouaria HadriKevin D. CostaRoger J. HajjarSima T. Tarzami
作者单位: 1Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
2Department of Physiology and Biophysics, College of Medicine, Howard University, Washington, DC 20060, USA
刊名: Stem Cell Research, 2017, Vol.23 , pp.77-86
来源数据库: Elsevier Journal
DOI: 10.1016/j.scr.2017.06.015
关键词: CXCR4CXCR7CardiogenesishiPSCEngineered tissue
英文摘要: Abstract(#br)CXCR4 and CXCR7 are prominent G protein-coupled receptors (GPCRs) for chemokine stromal cell-derived factor-1 (SDF-1/CXCL12). This study demonstrates that CXCR4 and CXCR7 induce differential effects during cardiac lineage differentiation and β-adrenergic response in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Using lentiviral vectors to ablate CXCR4 and/or CXCR7 expression, hiPSC-CMs were tested for phenotypic and functional properties due to gene knockdown. Gene expression and flow cytometry confirmed the pluripotent and cardiomyocyte phenotype of undifferentiated and differentiated hiPSCs, respectively. Although reduction of CXCR4 and CXCR7 expression resulted in a delayed cardiac phenotype, only knockdown of CXCR4 delayed the spontaneous beating...
全文获取路径: Elsevier  (合作)
影响因子:4.467 (2012)

  • lineage 血统
  • adrenergic 肾上腺素性的
  • distinct 相异的
  • specification 说瞄
  • cardiac 贲门
  • response 响应
  • tissue 组织