Simultaneous profiling of vitamin D metabolites in serum by supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS)
作者: Ting-Ting LiuLing-Zhi CheongQing-Qing ManXin ZhengJian ZhangShuang Song
作者单位: 1National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
2Department of Food Science and Technology, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, China
3Beijing Analysis Center, Shimadzu International Trade (China) Co., Beijing 100020, China
刊名: Journal of Chromatography B, 2019, Vol.1120 , pp.16-23
来源数据库: Elsevier Journal
DOI: 10.1016/j.jchromb.2019.04.050
关键词: Vitamin DMetabolitesSerumSupercritical fluid chromatography
英文摘要: Abstract(#br)Simultaneous profiling of serum vitamin D (VD) metabolites with similar structures is a big challenge. Thus, we developed and validated a SFC-MS/MS method, which is capable of eluting hydrophobic molecules, for quantification of VD 2 /VD 3 , 25-OH VD 2 /VD 3 , 3-epi-25-OH VD 2 /VD 3 , 1,25-(OH) 2 VD 2 /VD 3 and 24,25-(OH) 2 VD 2 /VD 3 . VD metabolites were extracted from human serum using acetonitrile solvent. Column stationary phase, elution gradients, flow rate, column temperature, ion-source type and buffer system in post-column make-up solvent were optimized. Baseline separation of 10 VD metabolites can be achieved using PFP column within 10 min; and detection performed under positive electrospray ionization mode allowed quantification of VD metabolites in serum matrix...
全文获取路径: Elsevier  (合作)
影响因子:2.487 (2012)

  • tandem 串联机
  • vitamin 维生素
  • chromatography 色层分离法
  • fluid 
  • spectrometry 光谱测定
  • supercritical 超临界的
  • profiling 仿形切削
  • serum 血清