White blood cell labeling with Technetium-99m ( 99m Tc) using red blood cell extracellular vesicles-mimetics
作者: Seung Hyun SonJi Min OhPrakash GangadaranHyun Dong JiHo Won LeeRamya Lakshmi RajendranSe Hwan BaekArunnehru GopalSenthilkumar KalimuthuShin Young JeongSang-Woo LeeJaetae LeeByeong-Cheol Ahn
作者单位: 1Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea
2Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea
3BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, School of Medicine, Kyungpook National University, Daegu, Republic of Korea
刊名: Blood Cells, Molecules and Diseases, 2020, Vol.80
来源数据库: Elsevier Journal
DOI: 10.1016/j.bcmd.2019.102375
关键词: InflammationWhite blood cellTechnetium-99mGamma cameraRed blood cellExtracellular vesicles-mimetics
英文摘要: Abstract(#br)Background(#br)Extracellular vesicles, have gained increasing attention for their application in drug delivery. Here, we developed a novel method for radiolabeling WBCs with 99m Tc using RBC-derived extracellular vesicles -mimetics (EVMs), and monitored in vivo inflammation tracking of 99m Tc-WBC using gamma camera in acute inflammation mouse model.(#br)Methods(#br)Engineered EVMs from RBCs were produced by a one-step extrusion method. RBC-EVMs were analyzed by NTA and TEM. Cells were labeled with 99m Tc by using 99m Tc-RBC-EVMs. Inflammation mice model was prepared and confirmed by 18 F-FDG PET/CT. 99m Tc-WBCs were injected in mice, and their biodistribution was analyzed by gamma camera.(#br)Finding(#br)The radiochemical purity of 99m Tc-RBC-EVMs was 100%. The 99m...
全文获取路径: Elsevier  (合作)
影响因子:2.259 (2012)

  • blood 血液
  • Tc 
  • White 怀特牌汽车
  • camera 摄影机
  • labeling 加标
  • extracellular 胞外的