Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution
作者: Veronika SvitkovaJana BlaskovicovaMonika TekelovaBrigitta Margit KallaiTeodora IgnatVeronika HorackovaPetr SkladalPavel KopelVojtech AdamDana FarkasovaJan Labuda
作者单位: 1Slovak University of Technology in Bratislava, Faculty of Chemical and Food Technology, Institute of Analytical Chemistry, Bratislava, Slovakia
2Masaryk University, Central European Institute of Technology, Brno, Czechia
3Mendel University in Brno, Brno University of Technology, Brno, Czechia
4Central European Institute of Technology, Brno University of Technology, Brno, Czechia
5Slovak Medical University in Bratislava, Bratislava, Slovakia
刊名: Sensors & Actuators: B. Chemical, 2017, Vol.243 , pp.435-444
来源数据库: Elsevier Journal
DOI: 10.1016/j.snb.2016.11.160
关键词: Nanoparticles toxicityQuantum dotsUV-C radiationDNA damageBiosensorBiosensing
原始语种摘要: Abstract(#br)Oxidation stress induced by nanoparticles is considered to be the main mechanism of their toxicity. An evaluation of oxidative damage to DNA, one of the mostly endangered molecules, belongs to general approaches used in nanomaterials cytotoxicology. However, for spherical, biochemical and other reasons, they should be differences in nanoparticles behaviour using tests based on cells and pure nucleic acid substances. The goal of the work is to detect structural damage to isolated salmon sperm double stranded DNA at its exposure to UV-C radiation (254nm) in the presence of thiol-capped CdS quantum dots of various size and fluorescence intensity. An electrochemical DNA-based biosensor composed of a glassy carbon electrode with a layered structure of chemical...
全文获取路径: Elsevier  (合作)
影响因子:3.535 (2012)

  • solution 溶液
  • irradiation 照射
  • chemical 化学的
  • DNA Deoxyribonucleic Acid
  • quantum 量子
  • degradation 减嚣夷酌
  • effect 效应
  • evaluation 评价
  • double 双重的
  • various 千变万化的