Ca 2+ Extrusion via Na + -Ca 2+ Exchangers in Rat Odontoblasts
作者: Maki TsumuraReijiro OkumuraShoko TatsuyamaHideki IchikawaTakashi MuramatsuToshio MatsudaAkemichi BabaKeiko SuzukiHiroshi KajiyaYoshinori SaharaMasayuki TokudaYasunori MomoseMasakazu TazakiMasaki ShimonoYoshiyuki Shibukawa
作者单位: 1Oral Health Science Center, Tokyo Dental College, Chiba, Japan
2Department of Physiology, Tokyo Dental College, Chiba, Japan
3Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Japan
4Department of Endodontics, Pulp and Periapical Biology, Tokyo Dental College, Chiba, Japan
5Department of Pathology, Tokyo Dental College, Chiba, Japan
6Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
7Laboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
8Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
9Southern Alberta Cancer Research Institute, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, AB, Canada
10Department of Physiological Science and Molecular Biology, Fukuoka Dental College, Fukuoka, Japan
11Department of Physiology, Tsurumi University School of Dental Medicine, Yokohama, Japan
12Department of Oral Physiology, School of Dentistry, Iwate Medical University, Morioka, Japan
刊名: Journal of Endodontics, 2010, Vol.36 (4), pp.668-674
来源数据库: Elsevier Journal
DOI: 10.1016/j.joen.2010.01.006
关键词: Ca 2+ signalingchannelintracellular Ca 2+ concentrationNa + -Ca 2+ exchangerodontoblastpatch-clamp
原始语种摘要: Abstract(#br)Introduction(#br)Intracellular Ca 2+ is essential to many signal transduction pathways, and its level is tightly regulated by the Ca 2+ extrusion system in the plasma membrane, which includes the Na + -Ca 2+ exchanger (NCX). Although expression of NCX1 isoforms has been demonstrated in odontoblasts, the detailed properties of NCX remain to be clarified. In this study, we investigated localization and ion-transporting/pharmacologic properties of NCX isoforms in rat odontoblasts.(#br)Methods(#br)We characterized both the reverse and forward modes of NCX activity in odontoblasts in a dental pulp slice preparation. Ca 2+ influx by reverse NCX activity was measured by fura-2 fluorescence. Ca 2+ efflux by forward NCX activity elicited inward Na + current as measured by...
全文获取路径: Elsevier  (合作)
影响因子:2.929 (2012)