Human sperm phosphoproteome reveals differential phosphoprotein signatures that regulate human sperm motility
作者: David Martin-HidalgoRebeca SerranoConcepcion ZaragozaLuis J. Garcia-MarinM. Julia Bragado
作者单位: 1Research Group of Intracellular Signalling and Technology of Reproduction (SINTREP), Institute of Biotechnology in Agriculture and Livestock (INBIO G+C), University of Extremadura, Caceres, Spain
2Unit for Multidisciplinary Research in Biomedicine (UMIB), Laboratory of Cell Biology, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal
3Department of Medicine and Animal Health, University of Extremadura, Caceres, Spain
刊名: Journal of Proteomics, 2020, Vol.215
来源数据库: Elsevier Journal
DOI: 10.1016/j.jprot.2020.103654
关键词: Human spermatozoaMotilityPhosphoproteomicProtein phosphorylationGlycogen shynthase kinase 3α
英文摘要: Abstract(#br)Human sperm motility is essential for fertilization and among pathologies underlying male infertility is asthenozoospermia. Nevertheless, mechanisms regulating sperm motility are not completely unraveled. This work investigates phosphoproteins underlying human sperm motility by using differential phosphoproteomic in two human sperm subpopulations: high (HM) and low (LM) motility, obtained by centrifugation in a density gradient. Phosphoproteomics (HPLC-MS/MS triple TOF), comparing human LM and HM phosphoproteomes, identified 210 phosphopeptides with different abundance that correspond with 119 sperm proteins. Analysis showed that 40% of phosphoproteins in LM spermatozoa are involved in metabolism, (catabolism, protein transport, lipid biosynthesis), 25% in spermatogenesis and...
全文获取路径: Elsevier  (合作)
影响因子:4.088 (2012)

  • sperm 精子
  • phosphoprotein 磷蛋白
  • motility 游动性
  • regulate 控制
  • human 人的
  • kinase 激酶
  • differential 差动的