Membrane-permeable Rab27A is a regulator of the acrosome reaction: Role of geranylgeranylation and guanine nucleotides
作者: Matías A. BustosOrnella LucchesiMaría C. RueteClaudia N. Tomes
作者单位: 1Instituto de Histologia y Embriologia de Mendoza (IHEM) Dr. Mario H. Burgos-CONICET, Universidad Nacional de Cuyo, casilla de correo 56, 5500 Mendoza, Argentina
刊名: Cellular Signalling, 2018, Vol.44 , pp.72-81
来源数据库: Elsevier Journal
DOI: 10.1016/j.cellsig.2018.01.010
关键词: Acrosome reactionPermeable proteinRab3Rab27ARap1
原始语种摘要: Abstract(#br)The acrosome reaction is the regulated exocytosis of mammalian sperm's single secretory granule, essential for fertilization. It relies on small GTPases, the cAMP binding protein Epac, and the SNARE complex, among other components. Here, we describe a novel tool to investigate Rab27-related signaling pathways: a hybrid recombinant protein consisting of human Rab27A fused to TAT, a cell penetrating peptide. With this tool, we aimed to unravel the connection between Rab3, Rab27 and Rap1 in sperm exocytosis and to deepen our understanding about how isoprenylation and guanine nucleotides influence the behaviour of Rab27 in exocytosis. Our results show that TAT-Rab27A-GTP-γ-S permeated into live sperm and triggered acrosomal exocytosis per se when geraylgeranylated but inhibited...
全文获取路径: Elsevier  (合作)
影响因子:4.304 (2012)

  • acrosome 顶体
  • reaction 反酌
  • protein 蛋白质
  • regulator 第器
  • guanine 鸟嘌呤
  • permeable 透水的