Multiple self-cleaning paper-based electrochemical ratiometric biosensor based on the inner reference probe and exonuclease III-assisted signal amplification strategy
作者: Xiaoxiao ZhengLi LiLina ZhangLi XieXianrang SongJinghua Yu
作者单位: 1School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China
2Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan, 250022, PR China
3Cancer Research Center, Shandong Tumor Hospital, Jinan, 250012, PR China
刊名: Biosensors and Bioelectronics, 2020, Vol.147
来源数据库: Elsevier Journal
DOI: 10.1016/j.bios.2019.111769
关键词: ElectrochemicalRatiometricPaper-based analytical deviceManganese super oxide dismutase geneInner reference element
英文摘要: Abstract(#br)Rapid and accurate detection of nucleic acids plays a major role in biological research and clinical diagnostics. Here, a 3D multiple self-cleaning electrochemical ratiometric microfluidic paper-based analytical device (SER-μPAD) has been constructed for manganese super oxide dismutase (MnSOD) gene detection on the basis of the inner reference probe and exonuclease Ш (Exo Ш)-assisted analytes recycling amplification method. To simplify manual operations, a multipath self-cleaning tab that could manipulate fluid transport was introduced into the paper-based device, realizing time-programmable self-cleaning of the electrode. For achieving sensitive detection of MnSOD gene, the methylene blue (MB)-modified capture probe (CP) as the inner reference element was first...
全文获取路径: Elsevier  (合作)
影响因子:5.437 (2012)

  • exonuclease 核酸外切酶
  • biosensor 生物传感器生物感测器
  • amplification 放大
  • reference 基准电压源
  • assisted 辅助的
  • paper 
  • based 基于
  • probe 探针
  • strategy 战略
  • inner 内的