Asn 229 in the third helix of VPAC 1 receptor is essential for receptor activation but not for receptor phosphorylation and internalization: Comparison with Asn 216 in VPAC 2 receptor
作者: Ingrid NachtergaelNathalie GaspardChristelle LangletPatrick RobberechtIngrid Langer
作者单位: 1Department of Biological Chemistry and Nutrition, Faculty of Medicine, Université Libre de Bruxelles, Belgium
刊名: Cellular Signalling, 2006, Vol.18 (12), pp.2121-2130
来源数据库: Elsevier Journal
DOI: 10.1016/j.cellsig.2006.03.006
关键词: VPAC 1 receptorVPAC 2 receptorTM helixG protein activationReceptor phosphorylationReceptor internalization
原始语种摘要: Abstract(#br)After stimulation with agonist, G protein coupled receptors (GPCR) undergo conformational changes that allow activation of G proteins to transduce the signal, followed by phosphorylation by kinases and arrestin binding to promote receptor internalization. Actual paradigm, based on a study of GPCR-A/rhodopsin family, suggests that a network of interactions between conserved residues located in transmembrane (TM) domains (mainly TM3, TM6 and TM7) is involved in the molecular switch leading to GPCR activation.(#br)We evaluated in CHO cells expressing the VPAC 1 receptor the role of the third transmembrane helix in agonist signalling by point mutation into Ala of the residues highly conserved in the secretin-family of receptors: Y 224 , N 229 , F 230 , W 232 , E 236 , G 237 , Y...
全文获取路径: Elsevier  (合作)
影响因子:4.304 (2012)

  • receptor 接受体
  • activation 活化
  • essential 本质的
  • helix 螺旋线
  • transmembrane 横跨膜的
  • protein 蛋白质
  • conformational 构造的
  • stimulated 受激
  • adenylate 腙甙酸盐
  • stimulation 激励