Effect of Spermine-NONOate on acrosome reaction and associated protein tyrosine phosphorylation in Murrah buffalo ( Bubalus bubalis ) spermatozoa
作者: R.A. SiddiqueS.K. Atreja
作者单位: 1Reproductive Biochemistry Laboratory, Animal Biochemistry Division, National Dairy Research Institute, Karnal 132001, Haryana, India
刊名: Animal Reproduction Science, 2012, Vol.131 (1-2), pp.81-87
来源数据库: Elsevier Journal
DOI: 10.1016/j.anireprosci.2012.02.010
关键词: Acrosome reactionBuffalo spermTyrosine phosphorylationNitric oxide
原始语种摘要: Abstract(#br)The present study was conducted to know the role of Nitric Oxide (NO) on the acrosome reaction (AR) in Murrah buffalo ( Bubalus bubalis ) spermatozoa. Ejaculated buffalo spermatozoa were washed, suspended in sp-TALP media containing 6mg BSA/mL and cell concentration was adjusted to 50×10 6 cells/mL. The cells were incubated for 6h in the absence or presence of heparin (10μg/mL) to induce capacitation. Fully capacitated spermatozoa were incubated in presence of 100μg/mL Lysophosphatidyl choline (LPC, T1) or 100μM Spermine-NONOate (T2) or 100mM l -NAME (T3) or 100μM...
全文获取路径: Elsevier  (合作)
分享到:
来源刊物:
影响因子:1.897 (2012)

×
关键词翻译
关键词翻译
  • spermatozoa 精子
  • acrosome 顶体
  • protein 蛋白质
  • reaction 反酌
  • associated 相关的
  • buffalo 水牛
  • tyrosine 酪氨酸
  • capacitation 获能
  • cAMP 环腺苷酸
  • significantly 较大地