Homogeneous label-free protein binding assay using small-molecule-labeled DNA nanomachine with DNAzyme-Based chemiluminescence detection
作者: Zhen ZhenJin-Wen LiuQian WenZhan WuJian-Hui Jiang
作者单位: 1Bao'an District Center for Disease Control and Prevention, Shenzhen, 518101, PR China
2State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China
3Key Laboratory of Phytochemical R&D of Hunan Province, and Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), Hunan Normal University, Changsha, 410081, PR China
4Department of Food Science and Technology, Central South University of Forestry and Technology, Changsha, 410004, PR China
刊名: Talanta, 2020, Vol.206
来源数据库: Elsevier Journal
DOI: 10.1016/j.talanta.2019.120175
关键词: Small molecule-protein interactionStrand displacement amplificationDNAzymeChemiluminescence
英文摘要: Abstract(#br)Detecting the interactions between small molecules and proteins was critical for disease theranostics and drug development. Here we propose a novel universal assay strategy for monitoring small molecule-protein interactions in solution using strand displacement amplification (SDA) mediated by protein binding to small molecule with DNAzyme-based chemiluminescence detection. The DNA polymerase and nicking enzyme assisted SDA could yield a great amount of peroxidase-mimicking DNAzyme sequences which cause significantly chemiluminescence signals, while protein binding to the small molecule label would prevent DNA polymerase from extending nick site and DNAzyme sequence, and thus the chemiluminescence signals would obviously decrease. This strategy was demonstrated using folate and...
全文获取路径: Elsevier  (合作)
影响因子:3.498 (2012)

  • detection 探测
  • protein 蛋白质
  • molecule 分子
  • label 标签标记
  • small 小的
  • binding 装订
  • assay 试金