Advances in enzyme substrate analysis with capillary electrophoresis
作者: Srikanth GattuCassandra L. CrihfieldGrace LuLloyd BwanaliLindsay M. VeltriLisa A. Holland
作者单位: 1C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506, United States
刊名: Methods, 2018
来源数据库: Elsevier Journal
DOI: 10.1016/j.ymeth.2018.02.005
关键词: Capillary electrophoresisEnzymeInhibitorMichaelis-Menten constant
英文摘要: Abstract(#br)Capillary electrophoresis provides a rapid, cost-effective platform for enzyme and substrate characterization. The high resolution achievable by capillary electrophoresis enables the analysis of substrates and products that are indistinguishable by spectroscopic techniques alone, while the small volume requirement enables analysis of enzymes or substrates in limited supply. Furthermore, the compatibility of capillary electrophoresis with various detectors makes it suitable for K M determinations ranging from nanomolar to millimolar concentrations. Capillary electrophoresis fundamentals are discussed with an emphasis on the separation mechanisms relevant to evaluate sets of substrate and product that are charged, neutral, and even chiral. The basic principles of...
全文获取路径: Elsevier  (合作)
影响因子:3.641 (2012)

  • capillary 毛状的
  • enzyme 
  • constant 常数
  • substrate 基质
  • analysis 分析