New insight into the Nox4 subcellular localization in HEK293 cells: First monoclonal antibodies against Nox4
作者: Leilei ZhangMinh Vu Chuong NguyenBernard LardyAlgirdas J. JesaitisAlexei GrichineFrancis RoussetMonique TalbotMarie-Hélène PacletGuanXiang QianFrançoise Morel
作者单位: 1GREPI Timc-Imag UMR-CNRS 5525, Enzymologie/CHU Albert Michallon, BP 217, 38043 Grenoble, France
2Department of Biochemistry and Molecular Biology, Research Center for Human Gene Therapy, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, PR China
3Department of Microbiology, Montana State University, Bozeman, MT 59717, USA
4Platform ‘Optical Microscopy – Cell Imaging’ – UJF, Inserm U823, Institut Albert Bonniot, 38076 La Tronche, France
5UMR-CNRS 8200, Institut de cancérologie Gustave Roussy, 94805 Villejuif, France
刊名: Biochimie, 2011, Vol.93 (3), pp.457-468
来源数据库: Elsevier Journal
DOI: 10.1016/j.biochi.2010.11.001
关键词: NADPH oxidase 4Monoclonal antibodiesSubcellular localizationO 2 −
英文摘要: Abstract(#br)Nox4, a member of Nox family of NADPH oxidase expressed in nonphagocytic cells, is a major source of reactive oxygen species in many cell types. But understanding of the role of Nox4 in the production of ROS and of regulation mechanism of oxidase activity is largely unknown. This study reports for the first time the generation and characterization of 5 mAbs against a recombinant Nox4 protein (AA: 206–578). Among 5 novel mAbs, 3 mAbs (8E9, 5F9, 6B11) specifically recognized Nox4 protein in HEK293 transfected cells or human kidney cortex by western blot analysis; mAb 8E9 reacted with intact tet-induced T-REx™ Nox4 cells in FACS studies. The other 2 mAbs 10B4 and 7C9 were shown to have a very weak reactivity after purification. Immunofluorescence confocal microscopy showed that...
全文获取路径: Elsevier  (合作)
影响因子:3.142 (2012)

  • monoclonal 单细胞系的
  • insight 识别力
  • against 反对
  • subcellular 亚细胞的
  • localization 定位
  • cells 麻风细胞