Independent prognostic value of ultra-sensitive quantification of tumor pre-treatment T790M subclones in EGFR mutated non-small cell lung cancer (NSCLC) treated by first/second generation TKI, depends on variant allele frequency (VAF): Results of the French cooperative thoracic intergroup (IFCT) biomarkers France project
作者: Michèle Beau-FallerErwan PencreachCharlotte LeducHélène BlonsJean-Philippe MerlioPierre-Paul BringuierFlorence de FraipontFabienne EscandeAntoinette LemoineL'Houcine OuafikMarc DenisPaul HofmanRoger LacaveSamia MelaabiAlexandra LanglaisPascale MissyFranck MorinDenis Moro-SibilotFabrice BarlesiJacques Cadranel
作者单位: 1Laboratory of Biochemistry and Molecular Biology, Centre Hospitalier Universitaire de Strasbourg, Hôpital de Hautepierre, Strasbourg, France
2IRFAC UMR-S1113, Inserm, Université de Strasbourg, Strasbourg, France
3Chest Department, Centre Hospitalier Universitaire de Strasbourg, Nouvel Hôpital Civil, Strasbourg, France
4HEGP, Biochimie UF de Pharmacogénétique et Oncologie Moléculaire, Paris, France
5Department of Pathology and Tumor Biology, CHU and University Bordeaux, Hôpital Haut-Lévêque, Pessac, France
6Centre de Biologie et Pathologie Est, Service d’Anatomie et de Cytologie Pathologique, Hospices Civils de Lyon et Université Claude Bernard Lyon 1, Lyon, France
7UM de Génétique moléculaire: Maladies Héréditaires et Oncologie, Pôle de Biologie et Pathologie, CHU Grenoble Alpes, Grenoble, France
8Laboratoire de Biochimie et Biologie moléculaire, CHRU Lille, LILLE, France
9Biochimie et Oncogénétique INSERM UMR-S1193, Hôpital Paul Brousse, Hôpitaux Universitaires Paris-Sud, Villejuif, France
10Aix Marseille Uni
刊名: Lung Cancer, 2020, Vol.140 , pp.19-26
来源数据库: Elsevier Journal
DOI: 10.1016/j.lungcan.2019.10.013
关键词: Non-small cell lung cancer (NSCLC)EGFR mutationT790MVariant allele frequency (VAF)Droplet digital PCR (ddPCR)
原始语种摘要: Abstract(#br)Objectives(#br)T790M mutations in EGFR -mutated non-small cell lung cancer (NSCLC) account for nearly 50% of acquired resistance mechanisms to EGFR-TKIs. Earlier studies suggested that tumor T790M could also be detected in TKI-naïve EGFR -mutated NSCLC. The aim of the study is to assess the prevalence and clinical significance of quantification of tumor pre-treatment T790M subclones.(#br)Materials and methods(#br)We analyzed 366 EGFR -mutated NSCLC patients of the real-life IFCT Biomarkers France study with available pre-treatment formalin-fixed paraffin-embedded (FFPE) tumor DNA before treatment by first/second-generation EGFR-TKI. We used ultra-sensitive Droplet Digital Polymerase Chain Reaction (ddPCR) QX200 (BIO-RAD®, Hercules, CA, USA). All samples were tested in...
全文获取路径: Elsevier  (合作)
影响因子:3.392 (2012)

  • intergroup 团体之间的
  • prognostic 预测的
  • cooperative 合啄
  • thoracic 胸的
  • sensitive 灵敏的
  • cancer 癌症
  • treated 加工的
  • treatment 处理
  • tumor 肿胀
  • survival 生存