Flow cytometry to estimate the cell disruption yield and biomass release of Chlorella sp. during bead milling
作者: Emre GünerkenEls D'HondtMichel EppinkKathy ElstRene Wijffels
作者单位: 1Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium
2Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV Wageningen, Netherlands
刊名: Algal Research, 2017, Vol.25 , pp.25-31
来源数据库: Elsevier Journal
DOI: 10.1016/j.algal.2017.04.033
关键词: Microalgae cell disruption yieldEfficient analytical methodFlow cytometryBiomass release
英文摘要: Abstract(#br)A number of visual, chemical and fluorescence-based methods are generally employed for monitoring of algae cell growth, culture health and biomass concentration. These methods are often time-consuming, demand destructive and high volume sampling. Rapid, efficient, cost-effective and automated methods which facilitate high-throughput and non-destructive sampling would highly benefit microalgae biotechnology. It is known in literature that with flow cytometry it is possible to monitor microalgae growth and microalgae culture health. Flow cytometry, however, has not been used to estimate biomass release and cell disruption yield. In this study with representative cultures of Chlorella sp., flow cytometry data were generated by a long pass filter (>670...
全文获取路径: Elsevier  (合作)
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关键词翻译
关键词翻译
  • disruption 破裂
  • estimate 估计
  • yield 成品率
  • release 释放
  • analytical 分析的
  • biomass 生物量
  • cytometry 血细胞计数
  • milling 捏炼
  • during 在的期间
  • method 方法