Identification differential behavior of Gd@C 82 (OH) 22 upon interaction with serum albumin using spectroscopic analysis
作者: Xing LiuXiangxian YingYanli LiHua YangWanshan HaoMeilan Yu
作者单位: 1College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
2College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China
3College of Materials Science and Engineering, China Jiliang University, Hangzhou 310018, China
刊名: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2018, Vol.203 , pp.383-396
来源数据库: Elsevier Journal
DOI: 10.1016/j.saa.2018.05.125
关键词: FullerenolsSerum albuminSynchronous fluorescenceUV–Vis absorptionThree-dimensional fluorescence
英文摘要: Abstract(#br)The interaction between Gd@C 82 (OH) 22 and serum albumin (HSA and BSA) were investigated by spectroscopic analysis. From the characteristic feature of fluorescence quenching spectra at different temperatures, the inherent binding information including quenching mechanism, association constants, number of binding site, fraction of initial fluorescence and basic thermodynamic parameters were calculated. The binding of Gd@C 82 (OH) 22 to serum albumin caused strong quenching of protein intrinsic fluorescence and the structural changes of serum albumin. At lower concentrations, Gd@C 82 (OH) 22 was likely to rise fluorescence quenching of serum albumin through individual static quenching process by forming a ground-state complex, while dynamic and static coexisting quenching...
全文获取路径: Elsevier  (合作)

  • spectroscopic 光谱的
  • OH Over-the-Horizon
  • fluorescence 荧光
  • interaction 相互酌
  • serum 血清
  • albumin 白蛋白
  • absorption 吸收
  • dimensional 量纲的
  • analysis 分析
  • behavior 行为