Development of a surface plasmon resonance immunosensor and ELISA for 3-nitrotyrosine in human urine
作者: Qiyi HeYingshan ChenDing ShenXiping CuiChunguo ZhangHuiyi YangWenying ZhongSergei A. EreminYanxiong FangSuqing Zhao
作者单位: 1Department of Pharmaceutical Engineering, Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, People’s Republic of China
2Faculty of Chemistry, M. V. Lomonosov Moscow State University, 119991 Moscow, Russia
3National Research Technical University MISiS, 119049 Moscow, Russia
刊名: Talanta, 2019, Vol.195 , pp.655-661
来源数据库: Elsevier Journal
DOI: 10.1016/j.talanta.2018.11.110
关键词: 3-nitrotyrosineSurface plasmon resonance (SPR)ELISAHuman urine
原始语种摘要: Abstract(#br)3-Nitrotyrosine (3-NT) is thought to be a relevant biomarker of nitrosative stress which is associated with many inflammatory and chronic diseases. It is necessary to develop confidential method for specific and sensitive 3-NT detection. In this paper, on the basis of anti-3-NT specific antibody, we developed a label-free indirect competitive surface plasmon resonance (SPR) immunosensor and ELISA for the detection of 3-NT. Under the optimized conditions, the SPR immunosensor can obtain a linear range of 0.17–6.07 μg/mL and a limit of detection (LOD) of 0.12 μg/mL while the ELISA can reach 0.33–9.94 μg/mL and a LOD of 0.24 μg/mL.The selectivity of 3-NT was also testified by six kinds of amino acid analogues. Besides, the developed SPR immunosensor was compared thoroughly with...
全文获取路径: Elsevier  (合作)
影响因子:3.498 (2012)

  • immunosensor 免疫感受器
  • plasmon 细胞质基因
  • resonance 共振
  • ELISA 酶联免疫吸收分析
  • urine 尿
  • confidential 秘密的
  • nitrotyrosine 硝基酷氨酸
  • surface 
  • sensitivity 灵敏度
  • developed 开发