Ectopic expression of an Eriobotrya japonica APETALA3 ortholog rescues the petal and stamen identities in Arabidopsis ap3-3 mutant
作者: Danlong JingWeiwei ChenMin ShiDan WangYan XiaQiao HeJiangbo DangQigao GuoGuolu Liang
作者单位: 1Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Beibei, Chongqing, 400715, PR China
2Academy of Agricultural Sciences of Southwest University, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, PR China
3State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, PR China
刊名: Biochemical and Biophysical Research Communications, 2020, Vol.523 (1), pp.33-38
来源数据库: Elsevier Journal
DOI: 10.1016/j.bbrc.2019.11.177
关键词: Eriobotrya japonicaAPETALA3MADS-box geneFlower developmentEctopic expression
原始语种摘要: Abstract(#br)Apetala3(#br)( AP3 ) encodes a floral homeotic class B-function MADS-box protein and plays crucial roles in petal and stamen development. To better understand the functional roles of AP3 orthologs in Eriobotrya , we isolated and identified an AP3 ortholog, referred to as EjAP3 , from Eriobotrya japonica . Analyses of protein sequence and phylogenetic tree showed that the EjAP3 was assigned to the rosids euAP3 lineage and included a distinctive PI-derived and euAP3 motifs at the C-terminal domain. Subcellular localization of EjAP3 was determined to be in the nucleus. Expression analysis suggested that EjAP3 expression was restricted only in petals and stamens, but not in sepals and carpels. Importantly, during the floral development, EjAP3 expression level was the highest at...
全文获取路径: Elsevier  (合作)

  • mutant 突变体
  • japonica 海带
  • stamen 雄蕊
  • Arabidopsis 阿布属
  • expression 表示
  • petal 花瓣