Octanol/water partitioning simulation by reversed-phase high performance liquid chromatography for structurally diverse acidic drugs: Effect of n -octanol as mobile phase additive
作者: Costas GiaginisStamatios TheocharisAnna Tsantili-Kakoulidou
作者单位: 1Department of Pharmaceutical Chemistry, School of Pharmacy, University of Athens, Panepistimiopolis, Zografou, Athens 15771, Greece
2Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 Mikras Asias Street, Athens 11527, Greece
刊名: Journal of Chromatography A, 2007, Vol.1166 (1-2), pp.116-125
来源数据库: Elsevier Journal
DOI: 10.1016/j.chroma.2007.08.004
关键词: Acidic drugsReversed-phase chromatographyn -Octanol as mobile phase additiveLipophilicityPolar interactions
原始语种摘要: Abstract(#br)The role of n -octanol as mobile phase additive for the lipophilicity assessment of 45 structurally diverse acidic drugs both at neutral (pH 2.5) and ionized form (pH 7.4) was investigated. Extrapolated retention factors log k w were determined on a BDS C18 column using methanol as organic modifier and different amounts of n -octanol as mobile phase additive. For more polar compounds, the effect of n -octanol in retention was found to decrease as their lipophilicity increased. In the case of carboxylic acids and oxicams, the differentiation in retention, in presence and absence of n -octanol, could be further attributed to the attenuation of polar interactions, concerning mainly hydrogen bonding. At pH 2.5, the use of n -octanol saturated buffer, without...
全文获取路径: Elsevier  (合作)
影响因子:4.612 (2012)

  • octanol 辛醇
  • structurally 在结构上
  • additive 添加剂
  • phase 相位
  • chromatography 色层分离法
  • mobile 可动的
  • retention 保留
  • reversed 倒转的
  • liquid 液体
  • performance 性能