TIMP-1/MMP-9 imbalance in an EBV-immortalized B lymphocyte cellular model: evidence for TIMP-1 multifunctional properties
作者: Philippe GaudinCandice TrocméSylvie BerthierSylvie KiefferJean BoutonnatChristophe LamyAnny SurlaJérome GarinFrançoise Morel
作者单位: 1Service de Rhumatologie, CHU A. Michallon, 38043 Grenoble Cedex 9, France
2GREPI, EA 2938-MENRT, Laboratoire d’Enzymologie, CHU A. Michallon, BP 217, 38043 Grenoble Cedex 9, France
3Laboratoire de Chimie des Protéines, DBMS, CEA, 38059 Grenoble Cedex 9, France
4Laboratoire de Cytologie, CHU A. Michallon, 38043 Grenoble Cedex 9, France
刊名: BBA - Molecular Cell Research, 2000, Vol.1499 (1), pp.19-33
来源数据库: Elsevier Journal
DOI: 10.1016/S0167-4889(00)00084-7
关键词: B lymphocyteMMP-9TIMP-1Growth factorApoptosis
原始语种摘要: Abstract(#br)Tissue inhibitors of metalloproteinases (TIMPs) were initially described as agents controlling metalloproteinase activity. The purpose of this study was to investigate the expression and the roles of TIMP-1 secreted by Epstein–Barr-virus (EBV)-immortalized B lymphocytes. TIMP-1 was isolated from conditioned medium of interleukin (IL)-1β stimulated EBV-B lymphocytes; purified TIMP-1 was identified by mass spectrometry and immunochemistry. TIMP-1-free MMP-9 was quantified after purification by zymography and enzyme-linked immunosorbent assay. EBV-B lymphocyte-secreted TIMP-1 inhibited MMP-9 gelatinolytic activity resulting in decreased B-cell transmigration as measured in vitro. The release of huge amounts of TIMP-1 in proportion to MMP-9 from B lymphocytes after EBV...
全文获取路径: Elsevier  (合作)

  • lymphocyte 淋巴细胞
  • EBV Extended Binary Vectors
  • imbalance 不稳定
  • suggestive 暗示的
  • immunosorbent 免疫吸收剂
  • interleukin 白细胞间介素
  • transmigration 移居
  • gelatinolytic 液化明胶的
  • inhibited 被禁止
  • factor 因素