Dual-mode detection of avian influenza virions (H9N2) by ICP-MS and fluorescence after quantum dot labeling with immuno-rolling circle amplification
作者: Guangyang XiaoBeibei ChenMan HeBin Hu
作者单位: 1Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China
刊名: Analytica Chimica Acta, 2020, Vol.1096 , pp.18-25
来源数据库: Elsevier Journal
DOI: 10.1016/j.aca.2019.10.063
关键词: H9N2 avian influenza virusRolling circle amplificationQuantum dotICP-MSDual mode detection
英文摘要: Abstract(#br)Avian influenza virus (AIVs), hosted in poultry, are the pathogens of many poultry diseases and human infections, which bring huge losses to the poultry breeding industry and huge panic to society. Therefore, it is of great significance to establish accurate and sensitive detection methods for AIVs. In this work, a dual-mode detection method based on immuno-rolling circle amplification (immuno-RCA) and quantum dots (QDs) labeling for inductively coupled plasma mass spectrometry (ICP-MS) and fluorescence detection of H9N2 AIV was developed. The dual-mode detection of the QDs by ICP-MS and fluorescence is used to achieve mutual verification within the analysis results, thus improving the accuracy of the method. With the immuno-RCA, the sensitivity of the method was increased by...
全文获取路径: Elsevier  (合作)
影响因子:4.387 (2012)

  • avian 鸟的
  • influenza 列性感冒
  • amplification 放大
  • immuno 免疫
  • rolling 
  • fluorescence 荧光
  • labeling 加标
  • circle 
  • detection 探测
  • quantum 量子