Brief Local Application of Progesterone via a Wearable Bioreactor Induces Long-Term Regenerative Response in Adult Xenopus Hindlimb
作者: Celia Herrera-RinconAnnie S. GoldingKristine M. MoranChristina HarrisonChristopher J. MartyniukJustin A. GuayJulia ZaltsmanHayley CarabelloDavid L. KaplanMichael Levin
作者单位: 1Biology Department and Allen Discovery Center, Tufts University, Medford, MA, USA
2Department of Chemical and Biological Engineering, Tufts University, Medford, MA, USA
3Center for Environmental and Human Toxicology and Department of Physiological Sciences, University of Florida, Gainesville, FL, USA
4Department of Biomedical Engineering, Tufts University, Medford, MA, USA
5Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA
刊名: Cell Reports, 2018, Vol.25 (6), pp.1593-1609.e7
来源数据库: Elsevier Journal
DOI: 10.1016/j.celrep.2018.10.010
关键词: SilkHydrogel deviceSpikeFrogBioelectricityRegenerationXenopusLimbBioreactor
原始语种摘要: Summary(#br)The induction of limb repair in adult vertebrates is a pressing, unsolved problem. Here, we characterize the effects of an integrated device that delivers drugs to severed hindlimbs of adult Xenopus laevis , which normally regenerate cartilaginous spikes after amputation. A wearable bioreactor containing a silk protein-based hydrogel that delivered progesterone to the wound site immediately after hindlimb amputation for only 24 hr induced the regeneration of paddle-like structures in adult frogs. Molecular markers, morphometric analysis, X-ray imaging, immunofluorescence, and behavioral assays were used to characterize the differences between the paddle-like structures of successful regenerates and hypomorphic spikes that grew in untreated animals. Our experiments establish a...
全文获取路径: Elsevier  (合作)
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关键词翻译
关键词翻译
  • regenerative 再生放大
  • amputation 切断术
  • characterize 成为...的特性
  • paddle 桨叶
  • hydrogel 水凝胶
  • regeneration 再生
  • progesterone 孕酮
  • normally 标准状态
  • regenerate 再生
  • viable 有生活力的