LncRNA NEAT1 reversed the hindering effects of miR-495-3p/STAT3 axis and miR-211/PI3K/AKT axis on sepsis-relevant inflammation
作者: Demeng XiaRenqi YaoPanyu ZhouChen WangYan XiaShuogui Xu
作者单位: 1Department of Emergency, Changhai Hospital, Naval Medical University, Shanghai, China
2Department of Burn, Changhai Hospital, Naval Medical University, Shanghai, China
刊名: Molecular Immunology, 2020, Vol.117 , pp.168-179
来源数据库: Elsevier Journal
DOI: 10.1016/j.molimm.2019.10.009
关键词: SepsisTraumaMice modelRAW264.7InflammationLncRNANEAT1MiR-495-3p/STAT3MiR-211/PI3K/AKT
原始语种摘要: Abstract(#br)Objective(#br)This investigation was intended to elucidate lncRNA-miRNA networks that could explain inflammation underlying sepsis progression.(#br)Methods(#br)In the first place, four kinds of mice models were established, namely, SHAM group (n = 30), trauma (TH) group (n = 30), lipopolysaccharide (LPS) group (n = 30) and TH + LPS group (n = 30). Their lung, spleen and liver tissues were gathered for determination of TNF-α, IL-6, IL-10 and MCP-1 levels. Furthermore, mouse mononuclear macrophage leukemia cell line (RAW264.7) was stimulated by LPS to establish inflammation cell models. Then si-NEAT1s, pcDNA3.1-NEAT1, miR-495-3p mimic, miR-495-3p inhibitor, miR-NC, miR-211 mimic and miR-211 inhibitor were, respectively, transfected into the cells, so as to observe the impacts...
全文获取路径: Elsevier  (合作)
影响因子:2.645 (2012)

  • inflammation 炎症
  • STAT 静止的
  • LPS Laboratory Peripheral System
  • reversed 倒转的
  • sepsis 败血症
  • cytokine 细胞因子
  • spleen 
  • mimic 毫米波单片集成电路
  • cells 麻风细胞
  • sponging 海绵体