Macrophage-targeted, enzyme-triggered fluorescence switch-on system for detection of embolism-vulnerable atherosclerotic plaques
作者: Yudai NaritaKosuke ShimizuKeisuke IkemotoRyuji UchinoMutsumi KosugiMarten B. MaessYasuhiro MagataNaoto OkuMikako Ogawa
作者单位: 1Department of Medical Biochemistry, Graduate Division of Pharmaceutical Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan
2Department of Molecular Imaging, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
3Laboratory of Bioanalytical Chemistry, Department of Medicinal Sciences, Faculty of Pharma-Science, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan
4Laboratory of Bioanalysis and Molecular Imaging, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12 Nishi 6, Kita-ku, Sapporo, Hokkaido 060-0812, Japan
刊名: Journal of Controlled Release, 2019, Vol.302 , pp.105-115
来源数据库: Elsevier Journal
DOI: 10.1016/j.jconrel.2019.03.025
关键词: Atherosclerotic vulnerable plaqueCathepsin BFluorescence imagingLiposomeMacrophagePhosphatidylserine
英文摘要: Abstract(#br)The development of atherosclerotic plaques is a critical step that can result in an arterial embolism. Therefore, detection of these vulnerable plaques is of clinical significance for the diagnosis of atherosclerosis. However, there are few imaging systems able to detect such plaques easily. In this study, we designed a new platform for near-infrared fluorescence (NIRF) imaging of macrophages in atherosclerotic plaques, one using both a liposomal DDS and an activatable fluorescent probe, and evaluated the utility of this imaging for the diagnosis of atherosclerosis. We first synthesized a fluorescent switch-on probe, Peptide-ICG2, which is optically silent under normal conditions but activated in the presence of the lysosomal enzyme, cathepsin B. To achieve...
全文获取路径: Elsevier  (合作)
影响因子:7.633 (2012)

  • vulnerable 要害
  • embolism 栓塞
  • targeted 对准目标的
  • triggered 触发的
  • fluorescence 荧光
  • detection 探测
  • imaging 图像形成
  • system 
  • Fluorescence 荧光
  • enzyme