Enhanced drought and salinity tolerance in transgenic mustard [Brassica juncea (L.) Czern & Coss.] overexpressing Arabidopsis group 4 late embryogenesis abundant gene (AtLEA4-1)
作者: Bedabrata SahaSagarika MishraJay Prakash AwasthiLingaraj SahooSanjib Kumar Panda
作者单位: 1Plant Molecular Biotechnology Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar-788011, India
2Department of Bioscience and Bioengineering, Indian Institute of Technology Guwahati, Guwahati-781039, India
刊名: Environmental and Experimental Botany, 2016, Vol.128 , pp.99-111
来源数据库: Elsevier Journal
DOI: 10.1016/j.envexpbot.2016.04.010
关键词: Brassica junceaLate Embryogenesis AbundantAgrobacterium mediatedDroughtSalinity
原始语种摘要: Abstract(#br)Indian mustard [ Brassica juncea (L.) Czern & Coss.] is one of the most important oilseed crop worldwide, yet relatively mild drought and salinity stress significantly reduce its growth and yield. Expression of LEA (Late Embryogenesis Abundant) proteins was previously shown to alleviate osmotic stress by stabilizing water status, protecting cytosolic structures and cell through increased membrane and protein stability. We overexpressed Arabidopsis group 4 LEA protein At LEA4-1 in mustard, resulting the transgenic lines to show enhanced drought and salinity tolerance. Southern hybridization showed the copy number of the gene inserted into the transgenics while, qRT PCR determined the transcript level of the overexpressed gene. Leaf senescence assay showed better adaptability...
全文获取路径: Elsevier  (合作)
影响因子:2.578 (2012)

  • abundant 丰富的
  • embryogenesis 胚发生
  • Arabidopsis 阿布属
  • mustard 芥末
  • drought 干旱
  • proteins 蛋白质
  • salinity 盐度
  • relative 相对的
  • increased 增加
  • inserted 嵌入的