Application of merged spectroscopic data combined with chemometric analysis for resolution of hemoglobin intermediates during chemical unfolding
作者: L. FotouhiS. YousefinejadN. SalehiA.A. SabouryN. SheibaniA.A. Moosavi-Movahedi
作者单位: 1Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
2Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran
3Department of Ophthalmology and Visual Sciences and McPherson Eye Research Institute, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA
刊名: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2015, Vol.136 , pp.1974-1981
来源数据库: Elsevier Journal
DOI: 10.1016/j.saa.2014.10.120
关键词: Folding intermediateChemical unfoldingChemometricsHemoglobinSpectroscopyCurve resolution
英文摘要: Abstract(#br)Using tetradecyltrimethylammonium bromide (TTAB) as a surfactant denaturant, and augmentation of different spectroscopic data, helped to detect the intermediates of hemoglobin (Hb) during unfolding process. UV–vis, fluorescence, and circular dichroism spectroscopy were used simultaneously to monitor different aspects of hemoglobin species from the tertiary or secondary structure points of view. Application of the multivariate curve resolution–alternating least square (MCR–ALS), using the initial estimates of spectral profiles and appropriate constraints on different parts of augmented spectroscopic data, showed good efficiency for characterization of intermediates during Hb unfolding. These results indicated the existence of five protein species, including three...
全文获取路径: Elsevier  (合作)

  • unfolding 展开
  • resolution 分解能力
  • spectroscopic 光谱的
  • intermediate 中频
  • Folding 可折叠的
  • combined 组合
  • hemoglobin 血红蛋白
  • analysis 分析
  • chemical 化学的
  • during 在的期间