Biochemical characterization of a novel surfactant-stable serine keratinase with no collagenase activity from Brevibacillus parabrevis CGMCC 10798
作者: Rong-Xian ZhangJin-Song GongChang SuDan-Dan ZhangHua TianWen-Fang DouHeng LiJin-Song ShiZheng-Hong Xu
作者单位: 1The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China
2National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China
3School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China
刊名: International Journal of Biological Macromolecules, 2016, Vol.93 , pp.843-851
来源数据库: Elsevier Journal
DOI: 10.1016/j.ijbiomac.2016.09.063
关键词: Brevibacillus parabrevisKeratinaseDehairing
原始语种摘要: Abstract(#br)Dehairing is a high pollution process in leather industry. Conventionally, the lime-sulfide mediated chemical process for dehairing would lead to the discharge of pollutants and corrosion of industrial equipment. Concerning these problems, keratinase has become a promising candidate for dehairing process in recent years. In this study, a keratinase-producing bacterium was isolated from sheepfold soil and identified as Brevibacillus parabrevis CGMCC 10798 based on the biochemical characteristics and molecular identification. The keratinase was purified to electrophoretic homogeneity with 17.19% of recovery, 13.18 folds of purification and an estimated molecular weight of 28kDa. The enzyme exhibited high keratinase activity and no collagenase activity....
全文获取路径: Elsevier  (合作)
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关键词翻译
关键词翻译
  • keratinase 角朊酶
  • activity 活度
  • serine 丝氨酸
  • stable 稳定的
  • novel 长篇小说
  • dehairing 精练桶
  • enzyme 
  • purified 净化的
  • process 过程
  • biochemical 生物化学的