Long-term leptin treatment exerts a pro-apoptotic effect on renal tubular cells via prostaglandin E 2 augmentation
作者: Yung-Ho HsuChung-Yi ChengYen-Cheng ChenTso-Hsiao ChenYuh-Mou SueWei-Lun TsaiCheng-Hsien Chen
作者单位: 1Division of Nephrology, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan
2Division of Nephrology, Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan
刊名: European Journal of Pharmacology, 2012, Vol.689 (1-3), pp.65-71
来源数据库: Elsevier Journal
DOI: 10.1016/j.ejphar.2012.06.008
关键词: LeptinGentamicinApoptosisRenal tubular cellsProstaglandin E2 (PGE 2 )
原始语种摘要: Abstract(#br)Adipokine leptin reportedly acts on the kidney in pathophysiological states. However, the influence of leptin on renal tubular epithelial cells is still unclear. Gentamicin, a widely used antibiotic for the treatment of bacterial infection, can cause nephrotoxicity. This study aims to investigate the influence of long-term leptin treatment on gentamicin-induced apoptosis in rat renal tubular cells (NRK-52E) and mice. We monitored apoptosis and molecular mechanisms using annexin V/ propidium iodide staining and small interfering RNA transfection. In NRK-52E cells, leptin reduced gentamicin-induced apoptosis at 24h, but significantly increased apoptosis at 48h. Long-term treatment of leptin decreased Bcl-x L expression and increased caspase...
全文获取路径: Elsevier  (合作)
影响因子:2.592 (2012)

  • tubular 管状的
  • cells 麻风细胞
  • treatment 处理
  • renal 肾的
  • gentamicin 艮他霉素
  • PGE Professional Group-Education
  • induced 感应的
  • prostaglandin 前列腺素
  • nephrotoxicity 中毒性肾损害
  • effect 效应