Hypomethylation of DNA and the insulin-like growth factor-II gene in dichloroacetic and trichloroacetic acid-promoted mouse liver tumors
作者: Lianhui TaoYingzhe LiPaula M KramerWei WangMichael A Pereira
作者单位: 1Department of Pathology, Medical College of Ohio, 3055 Arlington Avenue, Toledo, OH 43614-5806, USA
刊名: Toxicology, 2003, Vol.196 (1), pp.127-136
来源数据库: Elsevier Journal
DOI: 10.1016/j.tox.2003.11.011
关键词: Dichloroacetic acidDNA hypomethylationInsulin-like growth factor-II geneMouse liver tumorsmRNA expressionTrichloroacetic acid
原始语种摘要: Abstract(#br)Dichloroacetic acid (DCA) and trichloroacetic acid (TCA) are mouse liver carcinogens. DNA hypomethylation is a common molecular event in cancer that is induced by DCA and TCA. Hypomethylation of DNA and the insulin-like growth factor-II (IGF-II) gene was determined in DCA- and TCA-promoted liver tumors. Mouse liver tumors were initiated by N -methyl- N -nitrosourea and promoted by either DCA or TCA. By dot–blot analysis using an antibody for 5-methylcytosine, the DNA in DCA- and TCA-promoted tumors was demonstrated to be hypomethylated. The methylation status of 28 CpG sites in the differentially methylated region-2 (DMR-2) of mouse IGF-II gene was determined. In liver, 79.3±1.7% of the sites were methylated, while in DCA- and TCA-treated mice, only 46.4±2.1% and 58.0±1.7% of...
全文获取路径: Elsevier  (合作)
影响因子:4.017 (2012)

  • liver 
  • methylated 甲基化了的
  • mouse 小鼠
  • TCA TeleCommunications Association
  • insulin 胰岛素
  • growth 生长
  • exposed 被辐照的
  • methylation 甲基化酌
  • DCA Data Communications Administrator
  • carcinogens 致癌物