Development of an alpha-fetoprotein and Golgi protein 73 multiplex detection assay using xMAP technology
作者: Yun WuJie MaYipeng WangYonghong ZhangYongjin HouChunming ZhangHuanqin SunJianping SunZikang WangNing Li
作者单位: 1Department of Biomedical Information Center, Beijing You'an Hospital, Capital Medical University, Beijing, PR China
2Beijing Qingyuan Bio-Technologies Inc, Beijing, PR China
3Department of Prenatal Diagnosis Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, PR China
4Department of Surgery, Beijing You'an Hospital, Capital Medical University, Beijing, PR China
刊名: Clinica Chimica Acta, 2018, Vol.482 , pp.209-214
来源数据库: Elsevier Journal
DOI: 10.1016/j.cca.2018.03.039
关键词: LuminexXMAP technologyValidationMultiplex assayAlpha-fetoproteinGolgi protein 73
英文摘要: Abstracts(#br)Aim of the study(#br)Development of a new method to simultaneously detect Alpha-fetoprotein (AFP) and Golgi protein 73 (GP73) from peripheral blood.(#br)Material and methods(#br)Anti human AFP and GP73 monoclonal antibodies was used to develop a sandwich immunity reaction using xMAP technology for the simultaneous detection of plasma AFP and GP73. The assay evaluated the sensitivity, cross reactivity, range of detection, precision, recovery and linearity dilution effect. The assay utilized plasma samples and compared its performance with commercially available Enzyme Linked Immunosorbent Assay (ELISA) kits.(#br)Results(#br)The assay was successful in detecting AFP and GP73 simultaneously. Validation experiments demonstrated the limit of detection was AFP 0.006 μg/l and GP73...
全文获取路径: Elsevier  (合作)
影响因子:2.85 (2012)

  • fetoprotein 胎蛋白
  • multiplex 多倍仪
  • Golgi 高尔基
  • alpha 接字母顺序的
  • assay 试金
  • technology 工艺
  • Alpha α
  • detection 探测