Impact of methionine oxidation on the binding of human IgG1 to FcRn and Fcγ receptors
作者: Andrea Bertolotti-CiarletWeirong WangRebecca LownesPavlo PristatskyYulin FangTroy McKelveyYingzhe LiYunsong LiJames DrummondThomayant PrueksaritanontJosef Vlasak
作者单位: 1Bioprocess Analytical and Formulation Sciences, Merck & Co., Inc., West Point, PA, United States
2Drug Metabolism and Pharmacokinetics, Merck & Co., Inc., West Point, PA, United States
3Basic Research at Merck Research Laboratories, Merck & Co., Inc., West Point, PA, United States
刊名: Molecular Immunology, 2009, Vol.46 (8), pp.1878-1882
来源数据库: Elsevier Journal
DOI: 10.1016/j.molimm.2009.02.002
关键词: AntibodyFc fragmentMethionine oxidationFcRnFcγRFcγRIIa–131H
原始语种摘要: Abstract(#br)Methionine oxidation commonly occurs in the Fc fragment of therapeutic monoclonal antibodies; however, its impact on antibody function has not been addressed. Using surface plasmon resonance and cell binding assays, we examined the impact of methionine oxidation on the binding of two humanized IgG1 antibodies to Fcγ receptors (FcγR) and to the neonatal Fc receptor (FcRn). A panel of FcγRs, including FcγRI, FcγRIIa–131H, FcγRIIa–131R, FcγRIIb/c, FcγRIII ALF, FcγRIII ALV, and FcγRIIIb was evaluated. The binding of oxidized IgG1 molecules to individual receptors remained the same with the exception of FcγRIIa where a subtle decrease in binding to the 131H allele was observed. In contrast, but in agreement with recently reported structural changes associated with Met oxidation,...
全文获取路径: Elsevier  (合作)
影响因子:2.645 (2012)

  • oxidation 氧化
  • binding 装订
  • impact 冲击碰撞
  • values 价值观
  • reported 报告的
  • IgG 免疫球蛋白 G
  • constant 常数
  • chemical 化学的
  • monoclonal 单细胞系的
  • decrease 减少